Calcein AM
Catalog#: 1755-250 | Size: 5 x 50 µg
Catalog#: 1755-250 | Size: 5 x 50 µg
配製方法:
50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1Mm
取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,大約可以使用500次.
Calcein acetoxymethylester (Calcein AM) is a non-fluorescent, cell permeate compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein.
BIOVISION and TREVIGEN™ Calcein AM Fluorescent Dye can be used to fluorescently label viable cells for a variety of assays such as tumor cell invasion, endothelial cell migration, and endothelial cell tubulogenesis.
Alternate Name/Synonyms: Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester
Peptide sequence: N/A
Appearance: Off-white solid
Formulation: N/A
CAS Number: 148504-34-1
Molecular Formula: C₄₆H₄₆N₂O₂₃
Molecular Weight: 994.86
Purity: ≥95% by HPLC
Solubility: DMSO (5 mg/ml)
Storage Temp.: -20°C
Shipping Conditions: gel pack
Description: A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.
Handling: Protect from air and light
USAGE: For Research Use Only! Not For Use in Humans.
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原廠連結: http://www.trevigen.com/
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